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中科院海洋所科研人員利用ZooSCAN圖像掃描技術(shù)估算生物量
2020-01-09

ZooSCAN浮游動(dòng)物圖像掃描分析系統(tǒng)主要用于對(duì)液體中的浮游動(dòng)物樣品進(jìn)行計(jì)數(shù)、大小測(cè)量、種類鑒定以及生物量測(cè)定。

ZooSCAN系統(tǒng)是由ZooSCAN、ZooProcess和Plankton Identifer (PkID)等共同組成的,ZooSCAN是硬件部分,主要進(jìn)行浮游動(dòng)物樣品掃描,形成數(shù)字圖像。ZooProcess和PkID是軟件部分,分別以標(biāo)準(zhǔn)化的程序處理原始圖像、對(duì)不同個(gè)體的形態(tài)參數(shù)進(jìn)行自動(dòng)測(cè)量和對(duì)圖像中的浮游動(dòng)物進(jìn)行自動(dòng)分類和計(jì)數(shù)。其基本工作流程包括:1)掃描空白背景;2)掃描樣品,獲得原始圖片和元數(shù)據(jù)信息;3) 通過ZooProcess軟件,標(biāo)準(zhǔn)化原始圖片,提取并測(cè)量圖片中不同個(gè)體的形態(tài)參數(shù);4) 通過PkID 軟件建立圖像培訓(xùn)數(shù)據(jù)庫并進(jìn)行性能驗(yàn)證;5) 針對(duì)已掃描的樣品圖像,進(jìn)行浮游動(dòng)物的自動(dòng)識(shí)別,獲得不同類群浮游動(dòng)物的數(shù)量;6) 形態(tài)學(xué)參數(shù)的提取與分析,可進(jìn)一步獲得樣品的粒徑組成、生物學(xué)體積等信息。


應(yīng)用領(lǐng)域:生態(tài)學(xué)調(diào)查、漁業(yè)、水產(chǎn)養(yǎng)殖、教育。


近年,中國科學(xué)院海洋研究所科研人員利用ZooScan圖像掃描技術(shù),建立膠州灣浮游動(dòng)物優(yōu)勢(shì)種中華哲水蚤和強(qiáng)壯箭蟲體長、面積和體積與干重之間的回歸關(guān)系。比較了不同季節(jié)、不同參數(shù)之間的差異。結(jié)果表明,不同月份的回歸關(guān)系之間無顯著差異,在三個(gè)體型參數(shù)中,面積擬合生物量的程度最好,是利用圖像方法估算生物量最佳參數(shù)。中華哲水蚤和強(qiáng)壯箭蟲體型-生物量轉(zhuǎn)換關(guān)系可以分別表示為:lgDW=1.61lgA+1.69,lgDW=1.54lgA+1.32。


廠家對(duì)ZooSCAN進(jìn)行了全面的升級(jí)換代,鑒于工作需要,中國科學(xué)院海洋研究所再次選購1套ZooSCAN浮游動(dòng)物圖像掃描分析系統(tǒng),極大地增強(qiáng)了海洋所膠州灣生態(tài)環(huán)境研究的能力。


代表文獻(xiàn):

1.孫 松, 畢永坤, 孫曉霞,2013.基于圖像技術(shù)的膠州灣浮游動(dòng)物優(yōu)勢(shì)種體型參數(shù)與生物量轉(zhuǎn)換關(guān)系研究(RELATIONSHIP BETWEEN SHAPE PARAMETERS AND DRY WEIGHT OF THE DOMINANT ZOOPLANKTON IN JIAOZHOU BAY BASED ON IMAGE METHOD).海洋與湖沼(OCEANOLOGIA ET LIMNOLOGIA SINICA).44(1):15:22.

2.Pieter Vandromme, Lars Stemmann, Carmen Garcìa-Comas, Léo Berline, Xiaoxia Sun, Gaby Gorsky,2012.Assessing biases in computing size spectra of automatically classified zooplankton from imaging systems: A case study with the ZooScan integrated system.Methods in Oceanography.1–2:3–21.

3.Stéphanie Lelièvre, Elvire Antajan, and Sandrine Vaz,2012.Comparison of traditional microscopy and digitized image analysis to identify and delineate pelagic fish egg spatial distribution.Journal of Plankton Research.34(6):470-483.

4.Jesse R. Powell and Mark D. Ohman,2012.Use of glider-class acoustic Doppler profilers for estimating zooplankton biomass.Journal of Plankton Research.34(6):563-568.

5.Hirche, H., Alfred Wegener Inst. for Polar & Marine Res., Bremerhaven, Germany, Schulz, J., Hanken, T.,2012.A modular imaging system for collection and analysis of live and preserved zooplankton samples.OCEANS, 2012 - Yeosu.(1-4).

6.Lin Ye, Chun-Yi Chang, Chih-hao Hsieh,2011.Bayesian model for semi-automated zooplankton classification with predictive confidence and rapid category aggregation.Marine Ecology Progress Series.441:185-196.

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ECOLOGY STUDIES IN THE JIAOZHOU BAY)".海洋與湖沼(OCEANOLOGIA ET LIMNOLOGIA SINICA).42(5):647:653.

8.Gaby Gorsky, Mark D. Ohman, Marc Picheral, Stéphane Gasparini, Lars Stemmann, Jean-Baptiste Romagnan, Alison Cawood, Stéphane Pesant, Carmen García-Comas and Franck Prejger,2010.Digital zooplankton image analysis using the ZooScan integrated system.Journal of Plankton Research.32(3):285-303.

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Comas",2007.HARMONIZATION OF SAMPLING METHODS AND TREATMENT OF ZOOPLANKTON TIME SERIES.Rapp. Comm. int. Mer Médit., 38, 2007.490.

13.L. Stemmann, J.B. Romagnan, M.G. Mazzocchi, C. Garcia Comas, E. Antajan, M. Picheral, N.J. Daly Yahia, G. Gorsky,2007.ZOOPLANKTON COMMUNITY STRUCTURE AND SIZE DISTRIBUTION IN THE SOUTHERN TYRRHENIAN SEA DURING THE 2005 CIESM SUB 1 AND SUB 2 CRUISES.Rapp. Comm. int. Mer Médit., 38, 2007.606.

14."By Mark C. Benfi eld, Phi l ipp e Gros j ean, Phi l F. Culverhouse , Xabi er Irigoi en,

Michael E . Si eracki , An gel Lopez-Urrutia, Hans G. Dam, Qiao Hu,

Cabel l S . Davis , Al len Hansen, Cynthia H. Pi l skaln, Edward M. Riseman,

H oward Sc hultz, Paul E . Utgoff, and Gabri el Gorsky",2007.RAPID: research on automated plankton identification.Oceanography.20(2):172-187.

15.par Warembourg, C., Grosjean, Philippe, Picheral, M., Ibanez, F., Gorsky, G.,2005.Le ZOOSCAN: un système d’imagerie rapide pour la mesure et la classification automatique du zooplancton.Référence J. Recherche Océanographique, 30, (page 1-12) ..

16.Philippe Grosjean, Marc Picheral, Caroline Warembourg and Gabriel Gorsky,2004.Enumeration, measurement, and identification of net zooplankton samples using the ZOOSCAN digital imaging system.ICES Journal of Marine Science.61(4):518-525.

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